Global gene expression and focused knockout analysis reveals genes associated with fungal fruiting body development in Neurospora crassa.

ABSTRACT: Fungi can serve as highly tractable models for understanding genetic basis of sexual development in multicellular organisms. Applying a reverse-genetic approach to advance such a model, we used random and multitargeted primers to assay gene expression across perithecial development in Neurospora crassa. We found that functionally unclassified proteins accounted for most upregulated genes, whereas downregulated genes were enriched for diverse functions. Moreover, genes associated with developmental traits exhibited stage-specific peaks of expression. Expression increased significantly across sexual development for mating type gene mat a-1 and for mat A-1 specific pheromone precursor ccg-4. In addition, expression of a gene encoding a protein similar to zinc finger, stc1, was highly upregula

SUBMITTER: Wang Z 

PROVIDER: S-EPMC3910948 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature