Microbial biotransformation of gentiopicroside by the endophytic fungus Penicillium crustosum 2T01Y01.

ABSTRACT: Endophytic fungi are symbiotic with plants and possess multienzyme systems showing promising metabolite potency with region selectivity and stereoselectivity. The aim of this study was to use these special microorganisms as an in vitro model to mimic the potential mammalian metabolites of a natural iridoid gentiopicroside (GPS, compound 1). The fungi isolated from a medicinal plant, Dendrobium candidum Wall. ex Lindl., were screened for their biotransformation abilities with GPS as the substrate, and one strain with high converting potency was identified as Penicillium crustosum 2T01Y01 on the basis of the sequence of the internal transcribed spacer of the ribosomal DNA region. Upon the optimized incubation of P. crustosum 2T01Y01 with the substrate, seven deglycosylated metabolites were d

SUBMITTER: Zeng WL 

PROVIDER: S-EPMC3911023 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature