Amplification and purification of T4-like escherichia coli phages for phage therapy: from laboratory to pilot scale.

ABSTRACT: We investigated the amplification and purification of phage preparations with respect to titer, contamination level, stability, and technical affordability. Using various production systems (wave bags, stirred-tank reactors, and Erlenmeyer flasks), we obtained peak titers of 10(9) to 10(10) PFU/ml for T4-like coliphages. Phage lysates could be sterilized through 0.22-μm membrane filters without titer loss. Phages concentrated by differential centrifugation were not contaminated with cellular debris or bacterial proteins, as assessed by electron microscopy and mass spectrometry, respectively. Titer losses occurred by high-speed pelleting of phages but could be decreased by sedimentation through a sucrose cushion. Alternative phage concentration methods are prolonged medium-speed centrifugat

SUBMITTER: Bourdin G 

PROVIDER: S-EPMC3911048 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature