Development and bias assessment of a method for targeted metagenomic sequencing of marine cyanobacteria.

ABSTRACT: Prochlorococcus and Synechococcus are the most abundant photosynthetic organisms in oligotrophic waters and responsible for a significant percentage of the earth's primary production. Here we developed a method for metagenomic sequencing of sorted Prochlorococcus and Synechococcus populations using a transposon-based library preparation technique. First, we observed that the cell lysis technique and associated amount of input DNA had an important role in determining the DNA library quality. Second, we found that our transposon-based method provided a more even coverage distribution and matched more sequences of a reference genome than multiple displacement amplification, a commonly used method for metagenomic sequencing. We then demonstrated the method on Prochlorococcus and Synechococcus

SUBMITTER: Batmalle CS 

PROVIDER: S-EPMC3911227 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature