Clinical insights from metagenomic analysis of sputum samples from patients with cystic fibrosis.

ABSTRACT: As DNA sequencing becomes faster and cheaper, genomics-based approaches are being explored for their use in personalized diagnoses and treatments. Here, we provide a proof of principle for disease monitoring using personal metagenomic sequencing and traditional clinical microbiology by focusing on three adults with cystic fibrosis (CF). The CF lung is a dynamic environment that hosts a complex ecosystem composed of bacteria, viruses, and fungi that can vary in space and time. Not surprisingly, the microbiome data from the induced sputum samples we collected revealed a significant amount of species diversity not seen in routine clinical laboratory cultures. The relative abundances of several species changed as clinical treatment was altered, enabling the identification of the climax and att

SUBMITTER: Lim YW 

PROVIDER: S-EPMC3911355 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature