Newly identified phosphorylation site in the vesicular stomatitis virus P protein is required for viral RNA synthesis.

ABSTRACT: The vesicular stomatitis virus (VSV) RNA-dependent RNA polymerase consists of two viral proteins; the large (L) protein is the main catalytic subunit, and the phosphoprotein (P) is an essential cofactor for polymerase function. The P protein interacts with the L protein and the N-RNA template, thus connecting the polymerase to the template. P protein also binds to free N protein to maintain it in a soluble, encapsidation-competent form. Previously, five sites of phosphorylation were identified on the P protein and these sites were reported to be differentially important for mRNA synthesis or genomic replication. The previous studies were carried out by biochemical analysis of portions of the authentic viral P protein or by analysis of bacterium-expressed, exogenously phosphorylated P prote

SUBMITTER: Mondal A 

PROVIDER: S-EPMC3911598 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature