Insights into the complexity and functionality of hepatitis C virus NS5A phosphorylation.

ABSTRACT: The hepatitis C virus nonstructural NS5A protein has roles in genome replication, virus assembly, and modulation of host pathways. NS5A is a phosphoprotein, and it has been proposed that differential phosphorylation could regulate the various roles of the protein. By SDS-PAGE, two forms of NS5A with distinct apparent molecular weights can be observed, referred to as basally phosphorylated and hyperphosphorylated species. However, the sites of phosphorylation on these two species have not been unequivocally identified, hampering our understanding of the function and regulation of NS5A. To address this, we purified tagged NS5A from cells harboring a replicating subgenomic replicon and analyzed the purified protein by mass spectrometry. We identified six peptide fragments containing 12 phosph

SUBMITTER: Ross-Thriepland D 

PROVIDER: S-EPMC3911623 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature