Gene expression analysis in response to osmotic stimuli in the intervertebral disc with DNA microarray.

ABSTRACT:

Background

Intervertebral disc (IVD) cells experience a broad range of physicochemical stimuli under physiologic conditions, including alterations in their osmotic environment. At present, the molecular mechanisms underlying osmotic regulation in IVD cells are poorly understood. This study aims to screen genes affected by changes in osmotic pressure in cells of subjects aged 29 to 63 years old, with top-scoring pair (TSP) method.

Methods

Gene expression data set GSE1648 was downloaded from Gene Expression Omnibus database, including four hyper-osmotic stimuli samples, four iso-osmotic stimuli samples, and three hypo-osmotic stimuli samples. A novel, simple method, referred to as the TSP, was used in this study. Through this method, there was no need to perform data normaliza

SUBMITTER: Zhang W 

PROVIDER: S-EPMC3911967 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature