Validation of reference genes aiming accurate normalization of qRT-PCR data in Dendrocalamus latiflorus Munro.

ABSTRACT:

Background

Dendrocalamus latiflorus Munro distributes widely in subtropical areas and plays vital roles as valuable natural resources. The transcriptome sequencing for D. latiflorus Munro has been performed and numerous genes especially those predicted to be unique to D. latiflorus Munro were revealed. qRT-PCR has become a feasible approach to uncover gene expression profiling, and the accuracy and reliability of the results obtained depends upon the proper selection of stable reference genes for accurate normalization. Therefore, a set of suitable internal controls should be validated for D. latiflorus Munro.

Results

In this report, twelve candidate reference genes were selected and the assessment of gene expression stability was performed in ten tissue samples and four lea

SUBMITTER: Liu M 

PROVIDER: S-EPMC3911976 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature