Transcriptomic profiling of the development of the inflammatory response in human monocytes in vitro.

ABSTRACT: Monocytes/macrophages are key players in all phases of physiological and pathological inflammation. To understanding the regulation of macrophage functional differentiation during inflammation, we designed an in vitro model that recapitulates the different phases of the reaction (recruitment, initiation, development, and resolution), based on human primary blood monocytes exposed to sequential changes in microenvironmental conditions. All reaction phases were profiled by transcriptomic microarray analysis. Distinct clusters of genes were identified that are differentially regulated through the different phases of inflammation. The gene sets defined by GSEA analysis revealed that the inflammatory phase was enriched in inflammatory pathways, while the resolution phase comprised pathways rela

SUBMITTER: Italiani P 

PROVIDER: S-EPMC3912012 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature