Macrophage scavenger receptor A mediates the uptake of gold colloids by macrophages in vitro.

ABSTRACT:

Aims

While numerous studies have reported on nanoparticle uptake by phagocytic cells, the mechanisms of this uptake are poorly understood. A metastudy of research focusing on biological particulate matter has postulated that nanoparticles cannot be phagocytosed and therefore must enter cells via pinocytosis. The purpose of this study was to identify the route(s) of uptake of gold nanoparticles in vitro and to determine if these route(s) depend on particle size.

Materials & methods

The parent RAW264.7 cell line and its derivatives, transduced with a virus carrying siRNA to macrophage scavenger receptor A, were used as model phagocytes. Citrate-stabilized gold colloids were used as model nanoparticles. We used chemical inhibitors known to interfere with specific routes of part

SUBMITTER: Franca A 

PROVIDER: S-EPMC3912350 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature