Profiling deacetylase activities in cell lysates with peptide arrays and SAMDI mass spectrometry.

ABSTRACT: The development of arrays that can profile molecular activities in cells is important to understanding signaling pathways in normal and pathological settings. While oligonucleotide arrays are now routinely used to profile global gene expression, there is still a lack of tools for profiling enzyme activities in cell lysates. This paper describes the combination of peptide arrays formed on self-assembled monolayers and mass spectrometry to provide a label-free approach for identifying patterns of enzyme activities in cell lysates. The approach is demonstrated by profiling lysine deacetylase (KDAC) activities in cell lysates of the CHRF megakaryocytic (Mk) cell line. Class-specific deacetylase inhibitors were used to show that terminal Mk differentiation of CHRF cells is marked by a pronounce

SUBMITTER: Kuo HY 

PROVIDER: S-EPMC3912874 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature