Modeling pathogenesis of primary liver cancer in lineage-specific mouse cell types.

ABSTRACT:

Background & aims

Human primary liver cancer is classified into biologically distinct subgroups based on cellular origin. Liver cancer stem cells (CSCs) have been recently described. We investigated the ability of distinct lineages of hepatic cells to become liver CSCs and the phenotypic and genetic heterogeneity of primary liver cancer.

Methods

We transduced mouse primary hepatic progenitor cells, lineage-committed hepatoblasts, and differentiated adult hepatocytes with transgenes encoding oncogenic H-Ras and SV40LT. The CSC properties of transduced cells and their ability to form tumors were tested by standard in vitro and in vivo assays and transcriptome profiling.

Results

Irrespective of origin, all transduced cells acquired markers of CSC/progenitor cells, side p

SUBMITTER: Holczbauer A 

PROVIDER: S-EPMC3913051 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature