Prospective evaluation of methylated SEPT9 in plasma for detection of asymptomatic colorectal cancer.

ABSTRACT:

Background

As screening methods for colorectal cancer (CRC) are limited by uptake and adherence, further options are sought. A blood test might increase both, but none has yet been tested in a screening setting.

Objective

We prospectively assessed the accuracy of circulating methylated SEPT9 DNA (mSEPT9) for detecting CRC in a screening population.

Design

Asymptomatic individuals ≥50 years old scheduled for screening colonoscopy at 32 US and German clinics voluntarily gave blood plasma samples before colon preparation. Using a commercially available assay, three independent blinded laboratories assayed plasma DNA of all CRC cases and a stratified random sample of other subjects in duplicate real time PCRs. The primary outcomes measures were standardised for overall se

SUBMITTER: Church TR 

PROVIDER: S-EPMC3913123 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature