High-content, high-throughput screening for the identification of cytotoxic compounds based on cell morphology and cell proliferation markers.

ABSTRACT: Toxicity is a major cause of failure in drug discovery and development, and whilst robust toxicological testing occurs, efficiency could be improved if compounds with cytotoxic characteristics were identified during primary compound screening. The use of high-content imaging in primary screening is becoming more widespread, and by utilising phenotypic approaches it should be possible to incorporate cytotoxicity counter-screens into primary screens. Here we present a novel phenotypic assay that can be used as a counter-screen to identify compounds with adverse cellular effects. This assay has been developed using U2OS cells, the PerkinElmer Operetta high-content/high-throughput imaging system and Columbus image analysis software. In Columbus, algorithms were devised to identify changes in n

SUBMITTER: Martin HL 

PROVIDER: S-EPMC3914966 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature