Proteogenomic analysis of pathogenic yeast Cryptococcus neoformans using high resolution mass spectrometry.

ABSTRACT:

Background

Cryptococcus neoformans, a basidiomycetous fungus of universal occurrence, is a significant opportunistic human pathogen causing meningitis. Owing to an increase in the number of immunosuppressed individuals along with emergence of drug-resistant strains, C. neoformans is gaining importance as a pathogen. Although, whole genome sequencing of three varieties of C. neoformans has been completed recently, no global proteomic studies have yet been reported.

Results

We performed a comprehensive proteomic analysis of C. neoformans var. grubii (Serotype A), which is the most virulent variety, in order to provide protein-level evidence for computationally predicted gene models and to refine the existing annotations. We confirmed the protein-coding potential of 3,674 genes

SUBMITTER: Nagarajha Selvan LD 

PROVIDER: S-EPMC3915034 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature