Simple and sensitive detection of HBsAg by using a quantum dots nanobeads based dot-blot immunoassay.

ABSTRACT: Simple and sensitive detection of infectious disease at an affordable cost is urgently needed in developing nations. In this regard, the dot blot immunoassay has been used as a common protein detection method for detection of disease markers. However, the traditional signal reporting systems, such as those using enzymes or gold nanoparticles lack sensitivity and thus restrict the application of these methods for disease detection. In this study, we report a simple and sensitive detection method for the detection of infectious disease markers that couples the dot-blot immunoassay with quantum dots nanobeads (QDNBs) as a reporter. First, the QDNBs were prepared by an oil-in-water emulsion-evaporation technique. Because of the encapsulation of several QDs in one particle, the fluorescent sign

SUBMITTER: Zhang P 

PROVIDER: S-EPMC3915093 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature