Urinary EpCAM in urothelial bladder cancer patients: characterisation and evaluation of biomarker potential.

ABSTRACT:

Background

Epithelial cell adhesion molecule is overexpressed in bladder tumours and released from bladder cancer cells in vitro. We test the hypotheses that urinary EpCAM could act as a biomarker for primary bladder cancer detection and risk stratification.

Methods

Epithelial cell adhesion molecule was measured by ELISA in urine from 607 patients with primary bladder tumours and in urine from 53 non-cancer controls. Mann-Whitney tests and ROC analyses were used to determine statistical significance and discrimination between non-cancer controls and different stages and grades of disease. Multivariable modelling and Kaplan-Meier analyses were used to determine prognostic significance. The structure of urinary EpCAM was investigated by western blotting and mass spectrometry.<

SUBMITTER: Bryan RT 

PROVIDER: S-EPMC3915119 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature