TMPRSS4 regulates levels of integrin α5 in NSCLC through miR-205 activity to promote metastasis.

ABSTRACT:

Background

TMPRSS4 is a membrane-anchored protease involved in cell migration and invasion in different cancer types including lung cancer. TMPRSS4 expression is increased in NSCLC and its inhibition through shRNA reduces lung metastasis. However, molecular mechanisms leading to the protumorigenic regulation of TMPRSS4 in lung cancer are unknown.

Methods

miR-205 was identified as an overexpressed gene upon TMPRSS4 downregulation through microarray analysis. Cell migration and invasion assays and in vivo lung primary tumour and metastasis models were used for functional analysis of miR-205 overexpression in H2170 and H441 cell lines. Luciferase assays were used to identify a new miR-205 direct target in NSCLC.

Results

miR-205 overexpression promoted an epithelial pheno

SUBMITTER: Larzabal L 

PROVIDER: S-EPMC3915125 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature