First genome sequences of Achromobacter phages reveal new members of the N4 family.

ABSTRACT:

Background

Multi-resistant Achromobacter xylosoxidans has been recognized as an emerging pathogen causing nosocomially acquired infections during the last years. Phages as natural opponents could be an alternative to fight such infections. Bacteriophages against this opportunistic pathogen were isolated in a recent study. This study shows a molecular analysis of two podoviruses and reveals first insights into the genomic structure of Achromobacter phages so far.

Methods

Growth curve experiments and adsorption kinetics were performed for both phages. Adsorption and propagation in cells were visualized by electron microscopy. Both phage genomes were sequenced with the PacBio RS II system based on single molecule, real-time (SMRT) technology and annotated with several bioinform

SUBMITTER: Wittmann J 

PROVIDER: S-EPMC3915230 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature