Association of promoter methylation statuses of congenital heart defect candidate genes with Tetralogy of Fallot.

ABSTRACT:

Background

Although a lower methylation level of whole genome has been demonstrated in Tetralogy of Fallot (TOF) patients, little is known regarding changes in specific gene DNA methylation profiles and the possible associations with TOF. In current study, the promoter methylation statuses of congenital heart defect (CHD) candidate genes were measured in order to further understand epigenetic mechanisms that may play a role in the development of TOF.

Methods

The methylation levels of CHD candidate genes were measured using the Sequenom MassARRAY platform. QRT-PCR was used to analyze the mRNA levels of CHD candidate genes in the right ventricular myocardium of TOF cases and normal controls.

Results

Methylation status analysis was performed on the promoter regions of 71

SUBMITTER: Sheng W 

PROVIDER: S-EPMC3915623 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature