Identification of Maillard reaction products on peanut allergens that influence binding to the receptor for advanced glycation end products.

ABSTRACT:

Background

Recent immunological data demonstrated that dendritic cells preferentially recognize advanced glycation end product (AGE)-modified proteins, upregulate expression of the receptor for AGE (RAGE), and consequently bias the immune response toward allergy.

Methods

Peanut extract was characterized by mass spectrometry (MS) to elucidate the specific residues and specific AGE modifications found in raw and roasted peanuts and on rAra h 1 that was artificially glycated by incubation with glucose or xylose. The binding of the RAGE-V1C1 domain to peanut allergens was assessed by PAGE and Western analysis with anti-Ara h 1, 2, and 3 antibodies. IgE binding to rAra h 1 was also assessed using the same methods.

Results

AGE modifications were found on Ara h 1 and Ara h 3

SUBMITTER: Mueller GA 

PROVIDER: S-EPMC3915869 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature