Human TH17 cells express a functional IL-13 receptor and IL-13 attenuates IL-17A production.

ABSTRACT:

Background

IL-13 is a central mediator of airway responsiveness and mucus expression in patients with allergic airway inflammation, and IL-13 is currently a therapeutic target for asthma. However, little is known about how IL-13 regulates human CD4(+) T-cell lineages because IL-13 receptor (IL-13R) α1, a subunit of IL-13R, has not previously been reported to exist on human T cells.

Objective

We sought to determine whether human CD4(+) T(H)17 cells express IL-13Rα1 and whether IL-13 regulates T(H)17 cytokine production.

Methods

Naive human CD4(+) cells were isolated from whole blood, activated with anti-CD3 and anti-CD28, and polarized to T(H)1, T(H)2, T(H)17, or induced regulatory T cells in the presence of IL-13 (0-10 ng/mL). Cell supernatants, total RNA, or total pr

SUBMITTER: Newcomb DC 

PROVIDER: S-EPMC3916096 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature