A comparative study of lectin affinity based plant N-glycoproteome profiling using tomato fruit as a model.

ABSTRACT: Lectin affinity chromatography (LAC) can provide a valuable front-end enrichment strategy for the study of N-glycoproteins and has been used to characterize a broad range eukaryotic N-glycoproteomes. Moreover, studies with mammalian systems have suggested that the use of multiple lectins with different affinities can be particularly effective. A multi-lectin approach has also been reported to provide a significant benefit for the analysis of plant N-glycoproteins; however, it has yet to be determined whether certain lectins, or combinations of lectins are optimal for plant N-glycoproteome profiling; or whether specific lectins show preferential association with particular N-glycosylation sites or N-glycan structures. We describe here a comparative study of three mannose-binding lectins, co

SUBMITTER: Ruiz-May E 

PROVIDER: S-EPMC3916654 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature