The identification of novel potential injury mechanisms and candidate biomarkers in renal allograft rejection by quantitative proteomics.

ABSTRACT: Early transplant dysfunction and failure because of immunological and nonimmunological factors still presents a significant clinical problem for transplant recipients. A critical unmet need is the noninvasive detection and prediction of immune injury such that acute injury can be reversed by proactive immunosuppression titration. In this study, we used iTRAQ -based proteomic discovery and targeted ELISA validation to discover and validate candidate urine protein biomarkers from 262 renal allograft recipients with biopsy-confirmed allograft injury. Urine samples were randomly split into a training set of 108 patients and an independent validation set of 154 patients, which comprised the clinical biopsy-confirmed phenotypes of acute rejection (AR) (n = 74), stable graft (STA) (n = 74), chron

SUBMITTER: Sigdel TK 

PROVIDER: S-EPMC3916658 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature