Semiconductor-based DNA sequencing of histone modification states.

ABSTRACT: The recent development of a semiconductor-based, non-optical DNA sequencing technology promises scalable, low-cost and rapid sequence data production. The technology has previously been applied mainly to genomic sequencing and targeted re-sequencing. Here we demonstrate the utility of Ion Torrent semiconductor-based sequencing for sensitive, efficient and rapid chromatin immunoprecipitation followed by sequencing (ChIP-seq) through the application of sample preparation methods that are optimized for ChIP-seq on the Ion Torrent platform. We leverage this method for epigenetic profiling of tumour tissues.

SUBMITTER: Cheng CS 

PROVIDER: S-EPMC3917140 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature