Identification of cellular proteins that interact with human cytomegalovirus immediate-early protein 1 by protein array assay.

ABSTRACT: Human cytomegalovirus (HCMV) gene expression during infection is characterized as a sequential process including immediate-early (IE), early (E), and late (L)-stage gene expression. The most abundantly expressed gene at the IE stage of infection is the major IE (MIE) gene that produces IE1 and IE2. IE1 has been the focus of study because it is an important protein, not only for viral gene expression but also for viral replication. It is believed that IE1 plays important roles in viral gene regulation by interacting with cellular proteins. In the current study, we performed protein array assays and identified 83 cellular proteins that interact with IE1. Among them, seven are RNA-binding proteins that are important in RNA processing; more than half are nuclear proteins that are involved in g

SUBMITTER: Martinez FP 

PROVIDER: S-EPMC3917433 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature