Galectin-3 is associated with the plasma membrane of lens fiber cells.

ABSTRACT:

Purpose

To discover proteins that have the potential to contribute to the tight packing of fiber cells in the lens.

Methods

Crude fiber cell membranes were isolated from ovine lens cortex. Proteins were separated by two-dimensional gel electrophoresis, and selected protein spots identified by micro-sequencing. The identification of galectin-3 was confirmed by immunoblotting with a specific antibody. The association of galectin-3 with the fiber cell plasma membrane was investigated using immunofluorescence microscopy, solubilization trials with selected reagents, and immunoprecipitation to identify candidate ligands.

Results

A cluster of three protein spots with an apparent molecular weight of 31,000 and isoelectric points ranging between 7 and 8.5 were resolved and id

SUBMITTER: Gonen T 

PROVIDER: S-EPMC3917502 | biostudies-literature | 2000 Jan

REPOSITORIES: biostudies-literature