Expression of single-chain variable fragments fused with the Fc-region of rabbit IgG in Leishmania tarentolae.

ABSTRACT:

Background

In recent years the generation of antibodies by recombinant methods, such as phage display technology, has increased the speed by which antibodies can be obtained. However, in some cases when recombinant antibodies have to be validated, expression in E. coli can be problematic. This primarily occurs when codon usage or protein folding of specific antibody fragments is incompatible with the E. coli translation and folding machinery, for instance when recombinant antibody formats that include the Fc-region are needed. In such cases other expression systems can be used, including the protozoan parasite Leishmania tarentolae (L. tarentolae). This novel host for recombinant protein expression has recently shown promising properties for the expression of single-chain antibody

SUBMITTER: Jorgensen ML 

PROVIDER: S-EPMC3917567 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature