Energy balance-dependent regulation of ovine glucose 6-phosphate dehydrogenase protein isoform expression.

ABSTRACT: G6PDH is the rate-limiting enzyme of the pentose phosphate pathway and one of the principal source of NADPH, a major cellular reductant. Importantly, in ruminant's metabolism the aforementioned NADPH provided, is utilized for de novo fatty acid synthesis. Previous work of cloning the ovine (Ovis aries) og6pdh gene has revealed the presence of two cDNA transcripts (og6pda and og6pdb), og6pdb being a product of alternative splicing not similar to any other previously reported.(1) In the current study the effect of energy balance in the ovine G6PDH protein expression was investigated, shedding light on the biochemical features and potential physiological role of the oG6PDB isoform. Changes in energy balance leads to protein expression changes in both transcripts, to the opposite direction and

SUBMITTER: Triantaphyllopoulos KA 

PROVIDER: S-EPMC3917929 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature