Epigenetics meets genetics in acute myeloid leukemia: clinical impact of a novel seven-gene score.

ABSTRACT:

Purpose

Molecular risk stratification of acute myeloid leukemia (AML) is largely based on genetic markers. However, epigenetic changes, including DNA methylation, deregulate gene expression and may also have prognostic impact. We evaluated the clinical relevance of integrating DNA methylation and genetic information in AML.

Methods

Next-generation sequencing analysis of methylated DNA identified differentially methylated regions (DMRs) associated with prognostic mutations in older (≥ 60 years) cytogenetically normal (CN) patients with AML (n = 134). Genes with promoter DMRs and expression levels significantly associated with outcome were used to compute a prognostic gene expression weighted summary score that was tested and validated in four independent patient sets (n = 355

SUBMITTER: Marcucci G 

PROVIDER: S-EPMC3918538 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature