Solid State Fermentation of a Raw Starch Digesting Alkaline Alpha-Amylase from Bacillus licheniformis RT7PE1 and Its Characteristics.

ABSTRACT: The thermodynamic and kinetic properties of solids state raw starch digesting alpha amylase from newly isolated Bacillus licheniformis RT7PE1 strain were studied. The kinetic values Q p , Y p/s , Y p/X , and q p were proved to be best with 15% wheat bran. The molecular weight of purified enzyme was 112?kDa. The apparent K m and V max values for starch were 3.4?mg mL(-1) and 19.5?IU?mg(-1) protein, respectively. The optimum temperature and pH for ? -amylase were 55°C, 9.8. The half-life of enzyme at 95°C was 17h. The activation and denaturation activation energies were 45.2 and 41.2?kJ mol(-1), respectively. Both enthalpies (?H (?)) and entropies of activation (?S (?)) for denaturation of ? -amylase were lower than those reported for other thermostable ? -amylases.

SUBMITTER: Tabassum R 

PROVIDER: S-EPMC3918720 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature