Molecular divergence and species delimitation of the cultivated oyster mushrooms: integration of IGS1 and ITS.

ABSTRACT: Identification of edible mushrooms particularly Pleurotus genus has been restricted due to various obstacles. The present study attempted to use the combination of two variable regions of IGS1 and ITS for classifying the economically cultivated Pleurotus species. Integration of the two regions proved a high ability that not only could clearly distinguish the species but also served sufficient intraspecies variation. Phylogenetic tree (IGS1+ITS) showed seven distinct clades, each clade belonging to a separate species group. Moreover, the species differentiation was tested by AMOVA and the results were reconfirmed by presenting appropriate amounts of divergence (91.82% among and 8.18% within the species). In spite of achieving a proper classification of species by combination of IGS1 and ITS

SUBMITTER: Avin FA 

PROVIDER: S-EPMC3918722 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature