Toward rationally redesigning bacterial two-component signaling systems using coevolutionary information.

ABSTRACT: A challenge in molecular biology is to distinguish the key subset of residues that allow two-component signaling (TCS) proteins to recognize their correct signaling partner such that they can transiently bind and transfer signal, i.e., phosphoryl group. Detailed knowledge of this information would allow one to search sequence space for mutations that can be used to systematically tune the signal transmission between TCS partners as well as potentially encode a TCS protein to preferentially transfer signals to a nonpartner. Motivated by the notion that this detailed information is found in sequence data, we explore the sequence coevolution between signaling partners to better understand how mutations can positively or negatively alter their ability to transfer signal. Using direct coupling

SUBMITTER: Cheng RR 

PROVIDER: S-EPMC3918776 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature