Isoflurane post-treatment ameliorates GMH-induced brain injury in neonatal rats.

ABSTRACT:

Background and purpose

This study investigated whether isoflurane ameliorates neurological sequelae after germinal matrix hemorrhage (GMH) through activation of the cytoprotective sphingosine kinase/sphingosine-1-phosphate receptor/Akt pathway.

Methods

GMH was induced in P7 rat pups by intraparenchymal infusion of bacterial collagenase (0.3 U) into the right hemispheric germinal matrix. GMH animals received 2% isoflurane either once 1 hour after surgery or every 12 hours for 3 days. Isoflurane treatment was then combined with sphingosine-1-phosphate receptor-1/2 antagonist VPC23019 or sphingosine kinase 1/2 antagonist N,N-dimethylsphingosine.

Results

Brain protein expression of sphingosine kinase-1 and phosphorylated Akt were significantly increased after isoflurane p

SUBMITTER: Leitzke AS 

PROVIDER: S-EPMC3919515 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature