Longitudinal analysis of whole blood transcriptomes to explore molecular signatures associated with acute renal allograft rejection.

ABSTRACT: In this study, we explored a time course of peripheral whole blood transcriptomes from kidney transplantation patients who either experienced an acute rejection episode or did not in order to better delineate the immunological and biological processes measureable in blood leukocytes that are associated with acute renal allograft rejection. Using microarrays, we generated gene expression data from 24 acute rejectors and 24 nonrejectors. We filtered the data to obtain the most unambiguous and robustly expressing probe sets and selected a subset of patients with the clearest phenotype. We then performed a data-driven exploratory analysis using data reduction and differential gene expression analysis tools in order to reveal gene expression signatures associated with acute allograft rejection.

SUBMITTER: Shin H 

PROVIDER: S-EPMC3921155 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature