Concerted down-regulation of immune-system related genes predicts metastasis in colorectal carcinoma.

ABSTRACT:

Background

This study aimed at the identification of prognostic gene expression markers in early primary colorectal carcinomas without metastasis at the time point of surgery by analyzing genome-wide gene expression profiles using oligonucleotide microarrays.

Methods

Cryo-conserved tumor specimens from 45 patients with early colorectal cancers were examined, with the majority of them being UICC stage II or earlier and with a follow-up time of 41-115 months. Gene expression profiling was performed using Whole Human Genome 4x44K Oligonucleotide Microarrays. Validation of microarray data was performed on five of the genes in a smaller cohort.

Results

Using a novel algorithm based on the recursive application of support vector machines (SVMs), we selected a signature of 4

SUBMITTER: Fehlker M 

PROVIDER: S-EPMC3922093 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature