Comparison of three next-generation sequencing platforms for metagenomic sequencing and identification of pathogens in blood.

ABSTRACT:

Background

The introduction of benchtop sequencers has made adoption of whole genome sequencing possible for a broader community of researchers than ever before. Concurrently, metagenomic sequencing (MGS) is rapidly emerging as a tool for interrogating complex samples that defy conventional analyses. In addition, next-generation sequencers are increasingly being used in clinical or related settings, for instance to track outbreaks. However, information regarding the analytical sensitivity or limit of detection (LoD) of benchtop sequencers is currently lacking. Furthermore, the specificity of sequence information at or near the LoD is unknown.

Results

In the present study, we assess the ability of three next-generation sequencing platforms to identify a pathogen (viral or bac

SUBMITTER: Frey KG 

PROVIDER: S-EPMC3922542 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature