Specific regulation of low-abundance transcript variants encoding human Acyl-CoA binding protein (ACBP) isoforms.

ABSTRACT: Despite intensive efforts on annotation of eukaryotic transcriptoms, little is known about the regulation of low-abundance transcripts. To address this question, we analysed the regulation of novel low-abundance transcript variants of human acyl-CoA binding protein (ACBP), an important multifunctional housekeeping protein, which we have identified by screening of human expressed sequence tags in combination with ab initio gene prediction. By using RT-, real-time RT- and rapid amplification of cDNA ends-PCR in five human tissues, we find these transcripts, which are generated by a consequent use of alternative promoters and alternate first or first two exons, to be authentic ones. They show a tissue-specific distribution and intrinsic responsiveness to glucose and insulin. Promoter analyses

SUBMITTER: Nitz I 

PROVIDER: S-EPMC3922676 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature