Drawing networks of rejection - a systems biological approach to the identification of candidate genes in heart transplantation.

ABSTRACT: Technological development led to an increased interest in systems biological approaches to characterize disease mechanisms and candidate genes relevant to specific diseases. We suggested that the human peripheral blood mononuclear cells (PBMC) network can be delineated by cellular reconstruction to guide identification of candidate genes. Based on 285 microarrays (7370 genes) from 98 heart transplant patients enrolled in the Cardiac Allograft Rejection Gene Expression Observational study, we used an information-theoretic, reverse-engineering algorithm called ARACNe (algorithm for the reconstruction of accurate cellular networks) and chromatin immunoprecipitation assay to reconstruct and validate a putative gene PBMC interaction network. We focused our analysis on transcription factor (TF)

SUBMITTER: Cadeiras M 

PROVIDER: S-EPMC3922679 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature