EMS mutagenesis in mature seed-derived rice calli as a new method for rapidly obtaining TILLING mutant populations.

ABSTRACT:

Background

TILLING (Targeting Induced Local Lesions IN Genomes) is a reverse genetic method that combines chemical mutagenesis with high-throughput genome-wide screening for point mutation detection in genes of interest. However, this mutation discovery approach faces a particular problem which is how to obtain a mutant population with a sufficiently high mutation density. Furthermore, plant mutagenesis protocols require two successive generations (M1, M2) for mutation fixation to occur before the analysis of the genotype can begin.

Results

Here, we describe a new TILLING approach for rice based on ethyl methanesulfonate (EMS) mutagenesis of mature seed-derived calli and direct screening of in vitro regenerated plants. A high mutagenesis rate was obtained (i.e. one mutation

SUBMITTER: Serrat X 

PROVIDER: S-EPMC3923009 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature