An integrative strategy for quantitative analysis of the N-glycoproteome in complex biological samples.

ABSTRACT:

Background

The complexity of protein glycosylation makes it difficult to characterize glycosylation patterns on a proteomic scale. In this study, we developed an integrated strategy for comparatively analyzing N-glycosylation/glycoproteins quantitatively from complex biological samples in a high-throughput manner. This strategy entailed separating and enriching glycopeptides/glycoproteins using lectin affinity chromatography, and then tandem labeling them with 18O/16O to generate a mass shift of 6 Da between the paired glycopeptides, and finally analyzing them with liquid chromatography-mass spectrometry (LC-MS) and the automatic quantitative method we developed based on Mascot Distiller.

Results

The accuracy and repeatability of this strategy were first verified using stand

SUBMITTER: Wang J 

PROVIDER: S-EPMC3923275 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature