Identification of potential serum peptide biomarkers of biliary tract cancer using MALDI MS profiling.

ABSTRACT:

Background

The aim of this discovery study was the identification of peptide serum biomarkers for detecting biliary tract cancer (BTC) using samples from healthy volunteers and benign cases of biliary disease as control groups. This work was based on the hypothesis that cancer-specific exopeptidases exist and that their activities in serum can generate cancer-predictive peptide fragments from circulating proteins during coagulation.

Methods

This case control study used a semi-automated platform incorporating polypeptide extraction linked to matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) to profile 92 patient serum samples. Predictive models were generated to test a validation serum set from BTC cases and healthy volunteers.

Res

SUBMITTER: Sandanayake NS 

PROVIDER: S-EPMC3923428 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature