Potential cancer-related role of circadian gene TIMELESS suggested by expression profiling and in vitro analyses.

ABSTRACT:

Background

The circadian clock and cell cycle are two global regulatory systems that have pervasive behavioral and physiological effects on eukaryotic cells, and both play a role in cancer development. Recent studies have indicated that the circadian and cell cycle regulator, TIMELESS, may serve as a molecular bridge between these two regulatory systems.

Methods

To assess the role of TIMELESS in tumorigenesis, we analyzed TIMELESS expression data from publically accessible online databases. A loss-of-function analysis was then performed using TIMELESS-targeting siRNA oligos followed by a whole-genome expression microarray and network analysis. We further tested the effect of TIMELESS down-regulation on cell proliferation rates of a breast and cervical cancer cell line, as su

SUBMITTER: Mao Y 

PROVIDER: S-EPMC3924353 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature