Myogenic differentiation of muscular dystrophy-specific induced pluripotent stem cells for use in drug discovery.

ABSTRACT: Human induced pluripotent stem cells (iPSCs) represent a scalable source of potentially any cell type for disease modeling and therapeutic screening. We have a particular interest in modeling skeletal muscle from various genetic backgrounds; however, efficient and reproducible methods for the myogenic differentiation of iPSCs have not previously been demonstrated. Ectopic myogenic differentiation 1 (MyoD) expression has been shown to induce myogenesis in primary cell types, but the same effect has been unexpectedly challenging to reproduce in human iPSCs. In this study, we report that optimization of culture conditions enabled direct MyoD-mediated differentiation of iPSCs into myoblasts without the need for an intermediate step or cell sorting. MyoD induction mediated efficient cell fusion

SUBMITTER: Abujarour R 

PROVIDER: S-EPMC3925053 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature