In vivo protein interaction network identified with a novel real-time cross-linked peptide identification strategy.

ABSTRACT: Protein interaction topologies are critical determinants of biological function. Large-scale or proteome-wide measurements of protein interaction topologies in cells currently pose an unmet challenge that could dramatically improve understanding of complex biological systems. A primary impediment includes direct protein topology and interaction measurements from living systems since interactions that lack biological significance may be introduced during cell lysis. Furthermore, many biologically relevant protein interactions will likely not survive the lysis/sample preparation and may only be measured with in vivo methods. As a step toward meeting this challenge, a new mass spectrometry method called Real-time Analysis for Cross-linked peptide Technology (ReACT) has been developed that ena

SUBMITTER: Weisbrod CR 

PROVIDER: S-EPMC3925062 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature