Promoter hypermethylation of KLF4 inactivates its tumor suppressor function in cervical carcinogenesis.

ABSTRACT:

Objective

The KLF4 gene has been shown to be inactivated in cervical carcinogenesis as a tumor suppressor. However, the mechanism of KLF4 silencing in cervical carcinomas has not yet been identified. DNA methylation plays a key role in stable suppression of gene expression.

Methods

The methylation status of the KLF4 promoter CpG islands was analyzed by bisulfite sequencing (BSQ) in tissues of normal cervix and cervical cancer. KLF4 gene expression was detected by RT-PCR, immunohistochemistry and western blot. KLF4 promoter methylation in cervical cancer cell line was determined by BSQ and methylation-specific polymerase chain reaction (MS-PCR). Cell proliferation ability was detected by cell growth curve and MTT assay.

Results

The methylated allele was found in 41.90%

SUBMITTER: Yang WT 

PROVIDER: S-EPMC3925171 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature