Genome-wide gene expression profile analyses identify CTTN as a potential prognostic marker in esophageal cancer.

ABSTRACT:

Aim

Esophageal squamous cell carcinoma (ESCC) is one of the most common fatal malignances of the digestive tract. Its prognosis is poor mainly due to the lack of reliable markers for early detection and prognostic prediction. Here we aim to identify the molecules involved in ESCC carcinogenesis and those as potential markers for prognosis and as new molecular therapeutic targets.

Methods

We performed genome-wide gene expression profile analyses of 10 primary ESCCs and their adjacent normal tissues by cDNA microarrays representing 47,000 transcripts and variants. Candidate genes were then validated by semi quantitative reverse transcription-PCR (RT-PCR), tissue microarrays (TMAs) and immunohistochemistry (IHC) staining.

Results

Using an arbitrary cutoff line of signal

SUBMITTER: Lu P 

PROVIDER: S-EPMC3925182 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature