Transcriptome sequencing for high throughput SNP development and genetic mapping in Pea.

ABSTRACT:

Background

Pea has a complex genome of 4.3 Gb for which only limited genomic resources are available to date. Although SNP markers are now highly valuable for research and modern breeding, only a few are described and used in pea for genetic diversity and linkage analysis.

Results

We developed a large resource by cDNA sequencing of 8 genotypes representative of modern breeding material using the Roche 454 technology, combining both long reads (400 bp) and high coverage (3.8 million reads, reaching a total of 1,369 megabases). Sequencing data were assembled and generated a 68 K unigene set, from which 41 K were annotated from their best blast hit against the model species Medicago truncatula. Annotated contigs showed an even distribution along M. truncatula pseudochromosomes,

SUBMITTER: Duarte J 

PROVIDER: S-EPMC3925251 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature