Development of 5123 intron-length polymorphic markers for large-scale genotyping applications in foxtail millet.

ABSTRACT: Generating genomic resources in terms of molecular markers is imperative in molecular breeding for crop improvement. Though development and application of microsatellite markers in large-scale was reported in the model crop foxtail millet, no such large-scale study was conducted for intron-length polymorphic (ILP) markers. Considering this, we developed 5123 ILP markers, of which 4049 were physically mapped onto 9 chromosomes of foxtail millet. BLAST analysis of 5123 expressed sequence tags (ESTs) suggested the function for ∼71.5% ESTs and grouped them into 5 different functional categories. About 440 selected primer pairs representing the foxtail millet genome and the different functional groups showed high-level of cross-genera amplification at an average of ∼85% in eight millets and fiv

SUBMITTER: Muthamilarasan M 

PROVIDER: S-EPMC3925393 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature